Enzymatic Kinetics in Complex Media

This work is carried out in collaboration with Francesco Piazza and his group at the CBM and at the University of Florence. We study enzymatic (but not only) reactions in different complex media reminiscent to biological/cellular environments. These may interact with active components and significantly influence reaction rates or pathways. The interactions are investigated with appropriate biophysical methods involving a synchrotron radiation facility (Soleil).

Publications:

Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing
Marin Matić, Suman Saurabh, Josef Hamacek* , and Francesco Piazza*, J. Phys. Chem. B 2020, 124, 5, 727–734

Polyethylene glycol crowding effect on hyaluronidase activity monitored by capillary electrophoresis
Rouba Nasreddine, Lucija Orlic, Ghassan Al Hamoui Dit Banni, Syntia Fayad, Axel Marchal, Francesco Piazza, Chrystel Lopin-Bon, Josef Hamacek & Reine Nehmé
Analytical and Bioanalytical Chemistry volume 412, pages4195–4207(2020)

Fig. 1. Kinetic model of the late stage of HA degradation introduced in this paper — Kinetic theory of Hyaluronan Hydrolysis by Bovine Testicular Hyaluronidase in Standard and Crowded Environments
Reine Nehmé, Rouba Nasreddine, Lucija Orlic, Chrystel Lopin-Bon, Josef Hamacek, Francesco Piazza, Biochim Biophys Acta Gen Subj. 2020 Dec 27;1865(3):129837

Since 2021, we are involved in the project X-CROWD, which is financed by ANR. Our group is responsible of experimental biophysical investigations of enzymatic kinetics in crowded media. Actually, Josipa Cecic Vidos is hired for this work at the CBM.

Nations in the line of sight of international law 6 septembre 2024
As the International Criminal Court considers a request to issue an arrest warrant against Israel’s prime minister and three Hamas officials for crimes against humanity, Raphaëlle Nollez-Goldbach, a specialist in the subject, looks back on the very young history of international law.
A nose for smell disorders 3 septembre 2024
By depriving them more or less temporarily of their sense of smell, the Covid-19 pandemic made thousands of people abruptly realise the importance of their olfactory system. Research is now trying to decipher the causes of anosmia and to improve its treatment.
Planet 9, do you copy? 30 août 2024
For nearly ten years, astronomers have been trying to demonstrate the existence of a massive object thought to be orbiting in the outer reaches of the Solar System. Although the hypothesis is widely debated, a recent study claims that the absence of such a body is statistically impossible.